Biochemistry MCQ & Objective Questions
Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.
What You Will Practise Here
Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
Cellular respiration and photosynthesis: key processes in energy production.
Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
Genetic information flow: DNA replication, transcription, and translation.
Important biochemical equations and their applications in real-life scenarios.
Exam Relevance
Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.
Common Mistakes Students Make
Confusing the structures and functions of different biomolecules.
Misunderstanding metabolic pathways and their regulatory mechanisms.
Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
Overlooking the significance of enzyme kinetics in practical applications.
FAQs
Question: What are some effective ways to study Biochemistry for exams?Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.
Question: How can I improve my performance in Biochemistry MCQs?Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.
Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!
Q. Which process converts glucose into pyruvate?
A.
Glycolysis
B.
Krebs cycle
C.
Oxidative phosphorylation
D.
Fermentation
Show solution
Solution
Glycolysis is the metabolic pathway that converts glucose into pyruvate, producing ATP and NADH in the process.
Correct Answer:
A
— Glycolysis
Learn More →
Q. Which process directly produces ATP during fatty acid oxidation?
A.
Beta-oxidation
B.
Glycolysis
C.
Citric Acid Cycle
D.
Electron Transport Chain
Show solution
Solution
Beta-oxidation is the process that breaks down fatty acids to produce acetyl-CoA, which enters the citric acid cycle for ATP production.
Correct Answer:
A
— Beta-oxidation
Learn More →
Q. Which process ensures the accuracy of DNA replication?
A.
Transcription
B.
Proofreading
C.
Translation
D.
Splicing
Show solution
Solution
Proofreading by DNA polymerase ensures the accuracy of DNA replication by correcting errors.
Correct Answer:
B
— Proofreading
Learn More →
Q. Which process involves the conversion of DNA to RNA?
A.
Transcription
B.
Translation
C.
Replication
D.
Translocation
Show solution
Solution
Transcription is the process by which the information in a DNA sequence is copied into a complementary RNA sequence.
Correct Answer:
A
— Transcription
Learn More →
Q. Which process involves the decoding of mRNA to synthesize proteins?
A.
Transcription
B.
Translation
C.
Replication
D.
Splicing
Show solution
Solution
Translation is the process where ribosomes decode mRNA to synthesize proteins.
Correct Answer:
B
— Translation
Learn More →
Q. Which process involves the removal of an amino group from an amino acid?
A.
Transamination
B.
Deamination
C.
Decarboxylation
D.
Condensation
Show solution
Solution
Deamination is the process of removing an amino group from an amino acid, resulting in the formation of ammonia.
Correct Answer:
B
— Deamination
Learn More →
Q. Which process occurs in the mitochondria?
A.
Photosynthesis
B.
Glycolysis
C.
Krebs Cycle
D.
Fermentation
Show solution
Solution
The Krebs Cycle occurs in the mitochondria as part of cellular respiration.
Correct Answer:
C
— Krebs Cycle
Learn More →
Q. Which property of peptides is influenced by the side chains of amino acids?
A.
Primary structure
B.
Secondary structure
C.
Tertiary structure
D.
Quaternary structure
Show solution
Solution
The side chains (R groups) of amino acids influence the folding and interactions that determine the tertiary structure of peptides.
Correct Answer:
C
— Tertiary structure
Learn More →
Q. Which protein binds to the operator region of the lac operon?
A.
RNA polymerase
B.
Lac repressor
C.
cAMP receptor protein
D.
Beta-galactosidase
Show solution
Solution
The lac repressor binds to the operator region of the lac operon, preventing transcription of downstream genes when lactose is absent.
Correct Answer:
B
— Lac repressor
Learn More →
Q. Which protein is primarily involved in the unwinding of DNA during replication?
A.
DNA polymerase
B.
Helicase
C.
Ligase
D.
Topoisomerase
Show solution
Solution
Helicase is the enzyme responsible for unwinding the DNA double helix during replication, allowing access for other proteins.
Correct Answer:
B
— Helicase
Learn More →
Q. Which signaling pathway is primarily involved in cell growth and proliferation?
A.
MAPK/ERK pathway
B.
cAMP pathway
C.
JAK/STAT pathway
D.
Wnt pathway
Show solution
Solution
The MAPK/ERK pathway is primarily involved in regulating cell growth and proliferation.
Correct Answer:
A
— MAPK/ERK pathway
Learn More →
Q. Which solvent is commonly used in NMR spectroscopy to avoid interference?
A.
Water
B.
DMSO
C.
CDCl3
D.
Ethanol
Show solution
Solution
CDCl3 (deuterated chloroform) is commonly used as a solvent in NMR spectroscopy to minimize interference from solvent signals.
Correct Answer:
C
— CDCl3
Learn More →
Q. Which spectroscopy technique is best for identifying chemical bonds in a molecule?
A.
Mass Spectrometry
B.
Infrared Spectroscopy
C.
Nuclear Magnetic Resonance
D.
X-ray Crystallography
Show solution
Solution
Infrared spectroscopy is best for identifying chemical bonds in a molecule by measuring the absorption of infrared light at specific frequencies.
Correct Answer:
B
— Infrared Spectroscopy
Learn More →
Q. Which stage of photosynthesis occurs in the thylakoid membranes?
A.
Calvin Cycle
B.
Light-dependent reactions
C.
Glycolysis
D.
Krebs Cycle
Show solution
Solution
The light-dependent reactions of photosynthesis occur in the thylakoid membranes.
Correct Answer:
B
— Light-dependent reactions
Learn More →
Q. Which step of glycolysis involves substrate-level phosphorylation?
A.
Conversion of glucose to glucose-6-phosphate
B.
Conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate
C.
Conversion of phosphoenolpyruvate to pyruvate
D.
Conversion of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate
Show solution
Solution
The conversion of phosphoenolpyruvate to pyruvate involves substrate-level phosphorylation, producing ATP.
Correct Answer:
C
— Conversion of phosphoenolpyruvate to pyruvate
Learn More →
Q. Which step of the TCA cycle produces FADH2?
A.
Conversion of succinate to fumarate
B.
Conversion of isocitrate to alpha-ketoglutarate
C.
Conversion of malate to oxaloacetate
D.
Conversion of citrate to isocitrate
Show solution
Solution
The conversion of succinate to fumarate produces FADH2 in the TCA cycle.
Correct Answer:
A
— Conversion of succinate to fumarate
Learn More →
Q. Which structural feature is most critical for the stability of enzymes?
A.
Active site configuration
B.
Hydrophobic core
C.
Disulfide bonds
D.
All of the above
Show solution
Solution
All of these features contribute to the stability of enzymes.
Correct Answer:
D
— All of the above
Learn More →
Q. Which structure is characteristic of RNA?
A.
Double helix
B.
Single-stranded
C.
Triple helix
D.
Circular
Show solution
Solution
RNA is typically single-stranded, distinguishing it from the double helix structure of DNA.
Correct Answer:
B
— Single-stranded
Learn More →
Q. Which structure is involved in the detoxification of harmful substances in the liver?
A.
Smooth endoplasmic reticulum
B.
Rough endoplasmic reticulum
C.
Nucleus
D.
Mitochondria
Show solution
Solution
The smooth endoplasmic reticulum is involved in the detoxification of drugs and harmful substances in liver cells.
Correct Answer:
A
— Smooth endoplasmic reticulum
Learn More →
Q. Which structure is responsible for modifying and packaging proteins?
A.
Lysosome
B.
Golgi apparatus
C.
Nucleus
D.
Plasma membrane
Show solution
Solution
The Golgi apparatus modifies, sorts, and packages proteins for secretion or delivery to other organelles.
Correct Answer:
B
— Golgi apparatus
Learn More →
Q. Which technique is commonly used for the initial step in protein purification?
A.
Dialysis
B.
Precipitation
C.
Chromatography
D.
Electrophoresis
Show solution
Solution
Precipitation is often used as an initial step in protein purification to concentrate proteins and remove contaminants.
Correct Answer:
B
— Precipitation
Learn More →
Q. Which technique is commonly used to detect specific DNA sequences?
A.
Western blotting
B.
PCR
C.
ELISA
D.
Flow cytometry
Show solution
Solution
PCR (Polymerase Chain Reaction) is commonly used to detect and amplify specific DNA sequences.
Correct Answer:
B
— PCR
Learn More →
Q. Which type of antibody is most abundant in the bloodstream?
A.
IgA
B.
IgM
C.
IgG
D.
IgE
Show solution
Solution
IgG is the most abundant antibody in the bloodstream, playing a crucial role in the body's defense against infections.
Correct Answer:
C
— IgG
Learn More →
Q. Which type of antibody is produced first during an initial immune response?
A.
IgG
B.
IgA
C.
IgM
D.
IgE
Show solution
Solution
IgM is the first antibody produced in response to an infection, providing early defense before IgG levels rise.
Correct Answer:
C
— IgM
Learn More →
Q. Which type of antigen is recognized by T cells?
A.
Exogenous antigens
B.
Endogenous antigens
C.
Both exogenous and endogenous antigens
D.
None of the above
Show solution
Solution
T cells can recognize both exogenous and endogenous antigens.
Correct Answer:
C
— Both exogenous and endogenous antigens
Learn More →
Q. Which type of bond is primarily responsible for maintaining the tertiary structure of proteins?
A.
Hydrogen bonds
B.
Ionic bonds
C.
Disulfide bridges
D.
All of the above
Show solution
Solution
Tertiary structure is stabilized by hydrogen bonds, ionic bonds, and disulfide bridges.
Correct Answer:
D
— All of the above
Learn More →
Q. Which type of bond is primarily responsible for the interaction between proteins and nucleic acids?
A.
Ionic bonds
B.
Hydrogen bonds
C.
Covalent bonds
D.
Van der Waals forces
Show solution
Solution
Hydrogen bonds are crucial for the specific interactions between proteins and nucleic acids, allowing for the recognition of specific sequences.
Correct Answer:
B
— Hydrogen bonds
Learn More →
Q. Which type of bond is primarily responsible for the secondary structure of proteins?
A.
Ionic bonds
B.
Hydrogen bonds
C.
Disulfide bonds
D.
Peptide bonds
Show solution
Solution
Hydrogen bonds are primarily responsible for the formation of the secondary structure of proteins, such as alpha helices and beta sheets.
Correct Answer:
B
— Hydrogen bonds
Learn More →
Q. Which type of chromatography uses a solid stationary phase and a liquid mobile phase?
A.
Gas chromatography
B.
Liquid chromatography
C.
Thin-layer chromatography
D.
High-performance liquid chromatography
Show solution
Solution
Liquid chromatography uses a solid stationary phase and a liquid mobile phase for separation.
Correct Answer:
B
— Liquid chromatography
Learn More →
Q. Which type of detector is commonly used in HPLC?
A.
Mass spectrometer
B.
UV-Vis spectrophotometer
C.
Fluorescence detector
D.
All of the above
Show solution
Solution
All of the above detectors can be used in HPLC, with UV-Vis spectrophotometers being the most common.
Correct Answer:
D
— All of the above
Learn More →
Showing 361 to 390 of 412 (14 Pages)