Biochemistry MCQ & Objective Questions
Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.
What You Will Practise Here
Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
Cellular respiration and photosynthesis: key processes in energy production.
Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
Genetic information flow: DNA replication, transcription, and translation.
Important biochemical equations and their applications in real-life scenarios.
Exam Relevance
Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.
Common Mistakes Students Make
Confusing the structures and functions of different biomolecules.
Misunderstanding metabolic pathways and their regulatory mechanisms.
Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
Overlooking the significance of enzyme kinetics in practical applications.
FAQs
Question: What are some effective ways to study Biochemistry for exams?Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.
Question: How can I improve my performance in Biochemistry MCQs?Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.
Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!
Q. Which of the following is a limitation of UV luminance spectroscopy?
A.
High sensitivity to light
B.
Limited to visible light analysis
C.
Interference from other substances
D.
Requires expensive equipment
Show solution
Solution
A limitation of UV luminance spectroscopy is the potential interference from other substances present in the sample, which can affect accuracy.
Correct Answer:
C
— Interference from other substances
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Q. Which of the following is a primary application of FTIR spectroscopy in medicine?
A.
Blood glucose monitoring
B.
Tissue characterization
C.
X-ray imaging
D.
Ultrasound diagnostics
Show solution
Solution
FTIR spectroscopy is primarily used for tissue characterization, helping to identify chemical compositions and structures in biological samples.
Correct Answer:
B
— Tissue characterization
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Q. Which of the following is a property of water that is crucial for life?
A.
High density
B.
Low specific heat
C.
Universal solvent
D.
Inert nature
Show solution
Solution
Water is known as the universal solvent due to its ability to dissolve many substances.
Correct Answer:
C
— Universal solvent
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Q. Which of the following is a simple carbohydrate?
A.
Starch
B.
Glycogen
C.
Glucose
D.
Cellulose
Show solution
Solution
Glucose is a simple carbohydrate, also known as a monosaccharide.
Correct Answer:
C
— Glucose
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Q. Which of the following is an example of an allosteric inhibitor?
A.
ATP
B.
Citrate
C.
ADP
D.
NADH
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Solution
Citrate acts as an allosteric inhibitor of phosphofructokinase, regulating glycolysis.
Correct Answer:
B
— Citrate
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Q. Which of the following is NOT a characteristic of allosteric enzymes?
A.
Sigmoidal kinetics
B.
Cooperativity
C.
Michaelis-Menten kinetics
D.
Regulation by effectors
Show solution
Solution
Allosteric enzymes typically exhibit sigmoidal kinetics and do not follow Michaelis-Menten kinetics.
Correct Answer:
C
— Michaelis-Menten kinetics
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Q. Which of the following is NOT a characteristic of antigens?
A.
They can be proteins or polysaccharides
B.
They are always harmful to the body
C.
They can be recognized by the immune system
D.
They can be found on pathogens
Show solution
Solution
Not all antigens are harmful; some can be harmless substances that still provoke an immune response.
Correct Answer:
B
— They are always harmful to the body
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Q. Which of the following is NOT a characteristic of enzymes?
A.
They are proteins
B.
They are consumed in reactions
C.
They lower activation energy
D.
They are specific to substrates
Show solution
Solution
Enzymes are not consumed in reactions; they can be used repeatedly.
Correct Answer:
B
— They are consumed in reactions
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Q. Which of the following is NOT a characteristic of peptides?
A.
They are made of amino acids
B.
They can form complex three-dimensional structures
C.
They are always larger than proteins
D.
They can have biological activity
Show solution
Solution
Peptides are typically smaller than proteins and can range from just a few amino acids to several dozen.
Correct Answer:
C
— They are always larger than proteins
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Q. Which of the following is NOT a common type of gas chromatography?
A.
Capillary gas chromatography
B.
Packed column gas chromatography
C.
Supercritical fluid chromatography
D.
Gas-solid chromatography
Show solution
Solution
Supercritical fluid chromatography is not a type of gas chromatography; it uses supercritical fluids as the mobile phase.
Correct Answer:
C
— Supercritical fluid chromatography
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Q. Which of the following is NOT a component of a typical PCR reaction?
A.
Template DNA
B.
Primers
C.
Nucleotides
D.
RNA polymerase
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Solution
RNA polymerase is not used in PCR; instead, Taq polymerase is used for DNA synthesis.
Correct Answer:
D
— RNA polymerase
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Q. Which of the following is NOT a component of the adaptive immune system?
A.
B cells
B.
T cells
C.
Macrophages
D.
Antibodies
Show solution
Solution
Macrophages are part of the innate immune system, while B cells, T cells, and antibodies are components of the adaptive immune system.
Correct Answer:
C
— Macrophages
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Q. Which of the following is NOT a component of the genetic code?
A.
Amino acids
B.
Codons
C.
Nucleotides
D.
Proteins
Show solution
Solution
Proteins are the end products of gene expression, not a component of the genetic code itself.
Correct Answer:
D
— Proteins
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Q. Which of the following is NOT a component of the lac operon?
A.
Promoter
B.
Operator
C.
Repressor
D.
Enhancer
Show solution
Solution
The lac operon consists of a promoter, operator, and structural genes, but does not include an enhancer.
Correct Answer:
D
— Enhancer
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Q. Which of the following is NOT a component of the ribosome?
A.
rRNA
B.
tRNA
C.
Proteins
D.
mRNA
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Solution
mRNA is not a component of the ribosome; it is the template for translation.
Correct Answer:
D
— mRNA
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Q. Which of the following is NOT a function of RNA?
A.
Protein synthesis
B.
Genetic information storage
C.
Catalysis of biochemical reactions
D.
Regulation of gene expression
Show solution
Solution
RNA does not store genetic information; that function is primarily performed by DNA.
Correct Answer:
B
— Genetic information storage
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Q. Which of the following is NOT a product of glycolysis?
A.
NADH
B.
ATP
C.
FADH2
D.
Pyruvate
Show solution
Solution
FADH2 is not produced in glycolysis; it is produced in the citric acid cycle.
Correct Answer:
C
— FADH2
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Q. Which of the following is NOT a product of the TCA cycle?
A.
NADH
B.
FADH2
C.
ATP
D.
Glucose
Show solution
Solution
Glucose is not a product of the TCA cycle; it is a substrate for glycolysis.
Correct Answer:
D
— Glucose
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Q. Which of the following is NOT a source of carbohydrates?
A.
Fruits
B.
Vegetables
C.
Meat
D.
Grains
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Solution
Meat is not a source of carbohydrates; it is primarily a source of protein.
Correct Answer:
C
— Meat
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Q. Which of the following macromolecules can act as enzymes?
A.
Carbohydrates
B.
Lipids
C.
Proteins
D.
Nucleic acids
Show solution
Solution
Proteins can act as enzymes, catalyzing biochemical reactions.
Correct Answer:
C
— Proteins
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Q. Which of the following modifications can occur to mRNA before it is translated?
A.
5' capping
B.
3' polyadenylation
C.
RNA splicing
D.
All of the above
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Solution
All of the above modifications (5' capping, 3' polyadenylation, and RNA splicing) occur to mRNA before it is translated.
Correct Answer:
D
— All of the above
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Q. Which of the following mutations can lead to a change in the amino acid sequence?
A.
Silent mutation
B.
Missense mutation
C.
Nonsense mutation
D.
All of the above
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Solution
A missense mutation results in a different amino acid being incorporated into the protein, potentially altering its function.
Correct Answer:
B
— Missense mutation
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Q. Which of the following nucleotides is NOT found in RNA?
A.
Adenine
B.
Cytosine
C.
Thymine
D.
Uracil
Show solution
Solution
Thymine is found in DNA, while RNA contains uracil instead.
Correct Answer:
C
— Thymine
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Q. Which of the following processes occurs after transcription in eukaryotic cells?
A.
Translation
B.
Replication
C.
RNA splicing
D.
DNA repair
Show solution
Solution
RNA splicing is a process that occurs after transcription in eukaryotic cells, where introns are removed and exons are joined.
Correct Answer:
C
— RNA splicing
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Q. Which of the following statements about enzymes is true?
A.
Enzymes can work on any substrate
B.
Enzymes are specific to their substrates
C.
Enzymes do not require cofactors
D.
Enzymes are only found in humans
Show solution
Solution
Enzymes are specific to their substrates, meaning each enzyme typically catalyzes only one type of reaction.
Correct Answer:
B
— Enzymes are specific to their substrates
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Q. Which of the following statements about oxidative phosphorylation is true?
A.
It occurs in the cytoplasm
B.
It requires oxygen
C.
It produces lactic acid
D.
It is independent of the electron transport chain
Show solution
Solution
Oxidative phosphorylation requires oxygen as the final electron acceptor in the electron transport chain.
Correct Answer:
B
— It requires oxygen
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Q. Which of the following techniques is commonly used to determine the sequence of amino acids in a peptide?
A.
Nuclear magnetic resonance (NMR)
B.
Mass spectrometry
C.
X-ray crystallography
D.
Electrophoresis
Show solution
Solution
Mass spectrometry is a widely used technique for determining the sequence of amino acids in peptides.
Correct Answer:
B
— Mass spectrometry
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Q. Which of the following techniques is used to introduce recombinant DNA into host cells?
A.
Electroporation
B.
PCR
C.
Gel electrophoresis
D.
Western blotting
Show solution
Solution
Electroporation is a technique used to introduce recombinant DNA into host cells by applying an electrical field to increase cell permeability.
Correct Answer:
A
— Electroporation
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Q. Which organism is often used as a host for cloning in recombinant DNA technology?
A.
Bacteria
B.
Fungi
C.
Plants
D.
Animals
Show solution
Solution
Bacteria, particularly E. coli, are commonly used as hosts for cloning in recombinant DNA technology due to their rapid growth and ease of manipulation.
Correct Answer:
A
— Bacteria
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Q. Which pathway is activated during prolonged fasting to provide energy?
A.
Glycolysis
B.
Fatty acid oxidation
C.
Gluconeogenesis
D.
Pentose phosphate pathway
Show solution
Solution
During prolonged fasting, fatty acid oxidation is activated to provide energy from stored fat.
Correct Answer:
B
— Fatty acid oxidation
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