Biochemistry

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Biochemistry MCQ & Objective Questions

Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.

What You Will Practise Here

  • Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
  • Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
  • Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
  • Cellular respiration and photosynthesis: key processes in energy production.
  • Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
  • Genetic information flow: DNA replication, transcription, and translation.
  • Important biochemical equations and their applications in real-life scenarios.

Exam Relevance

Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.

Common Mistakes Students Make

  • Confusing the structures and functions of different biomolecules.
  • Misunderstanding metabolic pathways and their regulatory mechanisms.
  • Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
  • Overlooking the significance of enzyme kinetics in practical applications.

FAQs

Question: What are some effective ways to study Biochemistry for exams?
Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.

Question: How can I improve my performance in Biochemistry MCQs?
Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.

Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!

Q. Which enzyme is responsible for synthesizing RNA from a DNA template?
  • A. DNA polymerase
  • B. RNA polymerase
  • C. Ribosome
  • D. Ligase
Q. Which enzyme is responsible for the hydrolysis of lactose in E. coli?
  • A. Lactase
  • B. Beta-galactosidase
  • C. Lactose permease
  • D. Galactosidase
Q. Which factor can affect the resolution in HPLC?
  • A. Column length
  • B. Particle size of stationary phase
  • C. Flow rate
  • D. All of the above
Q. Which factor can affect the resolution of a gas chromatography separation?
  • A. Column length
  • B. Temperature of the detector
  • C. Type of carrier gas
  • D. All of the above
Q. Which factor can affect the yield of protein purification?
  • A. Temperature
  • B. pH
  • C. Choice of purification method
  • D. All of the above
Q. Which factor does NOT affect the separation in chromatography?
  • A. Temperature
  • B. Flow rate of the mobile phase
  • C. Nature of the stationary phase
  • D. Color of the sample
Q. Which hormone is primarily responsible for lowering blood glucose levels?
  • A. Glucagon
  • B. Insulin
  • C. Cortisol
  • D. Adrenaline
Q. Which immune cells are responsible for the destruction of virus-infected cells?
  • A. B cells
  • B. Helper T cells
  • C. Cytotoxic T cells
  • D. Regulatory T cells
Q. Which immune response is primarily activated by antigens?
  • A. Innate immune response
  • B. Adaptive immune response
  • C. Inflammatory response
  • D. Allergic response
Q. Which immunological technique is used to separate proteins based on their size?
  • A. Western blotting
  • B. Flow cytometry
  • C. Immunofluorescence
  • D. PCR
Q. Which immunological technique uses fluorescent dyes to visualize cells?
  • A. Immunofluorescence
  • B. ELISA
  • C. Western blotting
  • D. PCR
Q. Which level of protein structure is affected by denaturation?
  • A. Primary structure
  • B. Secondary structure
  • C. Tertiary structure
  • D. All of the above
Q. Which lipid is known as 'bad cholesterol'?
  • A. HDL
  • B. LDL
  • C. VLDL
  • D. Triglycerides
Q. Which macromolecule is involved in the storage of genetic information?
  • A. Proteins
  • B. Nucleic acids
  • C. Carbohydrates
  • D. Lipids
Q. Which macromolecule is primarily responsible for the structure of cell membranes?
  • A. Proteins
  • B. Nucleic acids
  • C. Carbohydrates
  • D. Lipids
Q. Which macromolecule serves as the primary source of energy for cells?
  • A. Proteins
  • B. Nucleic acids
  • C. Carbohydrates
  • D. Lipids
Q. Which metabolic pathway is primarily responsible for the detoxification of ammonia in the liver?
  • A. Glycolysis
  • B. Urea cycle
  • C. Krebs cycle
  • D. Fatty acid oxidation
Q. Which method can be used to determine the purity of a protein sample?
  • A. Mass spectrometry
  • B. Western blotting
  • C. Both A and B
  • D. None of the above
Q. Which mineral is a key antioxidant that helps protect cells from damage?
  • A. Selenium
  • B. Iron
  • C. Copper
  • D. Zinc
Q. Which mineral is crucial for maintaining healthy bones and teeth?
  • A. Sodium
  • B. Potassium
  • C. Calcium
  • D. Iron
Q. Which mineral is essential for the synthesis of collagen?
  • A. Zinc
  • B. Iron
  • C. Calcium
  • D. Sodium
Q. Which mineral is important for nerve transmission?
  • A. Calcium
  • B. Iron
  • C. Potassium
  • D. Sodium
Q. Which mineral is important for thyroid hormone production?
  • A. Iodine
  • B. Zinc
  • C. Calcium
  • D. Magnesium
Q. Which molecule acts as the electron carrier in glycolysis?
  • A. NAD+
  • B. FAD
  • C. CoA
  • D. ATP
Q. Which molecule carries amino acids to the ribosome during translation?
  • A. mRNA
  • B. tRNA
  • C. rRNA
  • D. DNA
Q. Which molecule is a key regulator of fatty acid oxidation?
  • A. Insulin
  • B. Glucagon
  • C. Citrate
  • D. Acetyl-CoA
Q. Which molecule is commonly involved as a second messenger in cell signaling?
  • A. ATP
  • B. cAMP
  • C. DNA
  • D. RNA
Q. Which molecule is produced as a byproduct of fatty acid oxidation?
  • A. Lactate
  • B. Acetyl-CoA
  • C. Glucose
  • D. Glycerol
Q. Which molecule is the primary energy currency of the cell?
  • A. Glucose
  • B. ATP
  • C. NADH
  • D. FADH2
Q. Which molecule is the starting substrate for the TCA cycle?
  • A. Pyruvate
  • B. Acetyl-CoA
  • C. Oxaloacetate
  • D. Citrate
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