Biochemistry MCQ & Objective Questions
Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.
What You Will Practise Here
Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
Cellular respiration and photosynthesis: key processes in energy production.
Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
Genetic information flow: DNA replication, transcription, and translation.
Important biochemical equations and their applications in real-life scenarios.
Exam Relevance
Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.
Common Mistakes Students Make
Confusing the structures and functions of different biomolecules.
Misunderstanding metabolic pathways and their regulatory mechanisms.
Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
Overlooking the significance of enzyme kinetics in practical applications.
FAQs
Question: What are some effective ways to study Biochemistry for exams?Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.
Question: How can I improve my performance in Biochemistry MCQs?Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.
Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!
Q. Which enzyme is responsible for synthesizing RNA from a DNA template?
A.
DNA polymerase
B.
RNA polymerase
C.
Ribosome
D.
Ligase
Show solution
Solution
RNA polymerase is the enzyme that synthesizes RNA by transcribing the DNA template during the process of transcription.
Correct Answer:
B
— RNA polymerase
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Q. Which enzyme is responsible for the hydrolysis of lactose in E. coli?
A.
Lactase
B.
Beta-galactosidase
C.
Lactose permease
D.
Galactosidase
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Solution
Beta-galactosidase is the enzyme that hydrolyzes lactose into glucose and galactose in E. coli.
Correct Answer:
B
— Beta-galactosidase
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Q. Which factor can affect the resolution in HPLC?
A.
Column length
B.
Particle size of stationary phase
C.
Flow rate
D.
All of the above
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Solution
All of the above factors can affect the resolution in HPLC, influencing the separation quality of analytes.
Correct Answer:
D
— All of the above
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Q. Which factor can affect the resolution of a gas chromatography separation?
A.
Column length
B.
Temperature of the detector
C.
Type of carrier gas
D.
All of the above
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Solution
All of the above factors can affect the resolution of a gas chromatography separation.
Correct Answer:
D
— All of the above
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Q. Which factor can affect the yield of protein purification?
A.
Temperature
B.
pH
C.
Choice of purification method
D.
All of the above
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Solution
All of the above factors can significantly affect the yield of protein purification.
Correct Answer:
D
— All of the above
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Q. Which factor does NOT affect the separation in chromatography?
A.
Temperature
B.
Flow rate of the mobile phase
C.
Nature of the stationary phase
D.
Color of the sample
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Solution
The color of the sample does not affect the separation in chromatography.
Correct Answer:
D
— Color of the sample
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Q. Which hormone is primarily responsible for lowering blood glucose levels?
A.
Glucagon
B.
Insulin
C.
Cortisol
D.
Adrenaline
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Solution
Insulin is the hormone that lowers blood glucose levels.
Correct Answer:
B
— Insulin
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Q. Which immune cells are responsible for the destruction of virus-infected cells?
A.
B cells
B.
Helper T cells
C.
Cytotoxic T cells
D.
Regulatory T cells
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Solution
Cytotoxic T cells are specialized in recognizing and destroying virus-infected cells.
Correct Answer:
C
— Cytotoxic T cells
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Q. Which immune response is primarily activated by antigens?
A.
Innate immune response
B.
Adaptive immune response
C.
Inflammatory response
D.
Allergic response
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Solution
The adaptive immune response is primarily activated by the presence of antigens.
Correct Answer:
B
— Adaptive immune response
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Q. Which immunological technique is used to separate proteins based on their size?
A.
Western blotting
B.
Flow cytometry
C.
Immunofluorescence
D.
PCR
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Solution
Western blotting is used to separate proteins based on their size and then detect specific proteins using antibodies.
Correct Answer:
A
— Western blotting
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Q. Which immunological technique uses fluorescent dyes to visualize cells?
A.
Immunofluorescence
B.
ELISA
C.
Western blotting
D.
PCR
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Solution
Immunofluorescence uses fluorescent dyes to visualize the presence and location of specific proteins or antigens in cells.
Correct Answer:
A
— Immunofluorescence
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Q. Which level of protein structure is affected by denaturation?
A.
Primary structure
B.
Secondary structure
C.
Tertiary structure
D.
All of the above
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Solution
Denaturation can affect all levels of protein structure, particularly secondary, tertiary, and quaternary structures.
Correct Answer:
D
— All of the above
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Q. Which lipid is known as 'bad cholesterol'?
A.
HDL
B.
LDL
C.
VLDL
D.
Triglycerides
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Solution
LDL (low-density lipoprotein) is often referred to as 'bad cholesterol' because it can lead to plaque buildup in arteries.
Correct Answer:
B
— LDL
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Q. Which macromolecule is involved in the storage of genetic information?
A.
Proteins
B.
Nucleic acids
C.
Carbohydrates
D.
Lipids
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Solution
Nucleic acids, such as DNA and RNA, are responsible for storing and transmitting genetic information.
Correct Answer:
B
— Nucleic acids
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Q. Which macromolecule is primarily responsible for the structure of cell membranes?
A.
Proteins
B.
Nucleic acids
C.
Carbohydrates
D.
Lipids
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Solution
Lipids, particularly phospholipids, form the structural basis of cell membranes.
Correct Answer:
D
— Lipids
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Q. Which macromolecule serves as the primary source of energy for cells?
A.
Proteins
B.
Nucleic acids
C.
Carbohydrates
D.
Lipids
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Solution
Carbohydrates are the primary source of energy for cells.
Correct Answer:
C
— Carbohydrates
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Q. Which metabolic pathway is primarily responsible for the detoxification of ammonia in the liver?
A.
Glycolysis
B.
Urea cycle
C.
Krebs cycle
D.
Fatty acid oxidation
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Solution
The urea cycle is primarily responsible for the detoxification of ammonia in the liver, converting it to urea.
Correct Answer:
B
— Urea cycle
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Q. Which method can be used to determine the purity of a protein sample?
A.
Mass spectrometry
B.
Western blotting
C.
Both A and B
D.
None of the above
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Solution
Both mass spectrometry and Western blotting can be used to assess the purity of a protein sample.
Correct Answer:
C
— Both A and B
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Q. Which mineral is a key antioxidant that helps protect cells from damage?
A.
Selenium
B.
Iron
C.
Copper
D.
Zinc
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Solution
Selenium is an important antioxidant that helps protect cells from oxidative stress.
Correct Answer:
A
— Selenium
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Q. Which mineral is crucial for maintaining healthy bones and teeth?
A.
Sodium
B.
Potassium
C.
Calcium
D.
Iron
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Solution
Calcium is vital for the development and maintenance of strong bones and teeth.
Correct Answer:
C
— Calcium
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Q. Which mineral is essential for the synthesis of collagen?
A.
Zinc
B.
Iron
C.
Calcium
D.
Sodium
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Solution
Zinc is important for collagen synthesis, which is vital for skin, bone, and connective tissue health.
Correct Answer:
A
— Zinc
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Q. Which mineral is important for nerve transmission?
A.
Calcium
B.
Iron
C.
Potassium
D.
Sodium
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Solution
Potassium is essential for nerve transmission and helps maintain proper nerve function.
Correct Answer:
C
— Potassium
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Q. Which mineral is important for thyroid hormone production?
A.
Iodine
B.
Zinc
C.
Calcium
D.
Magnesium
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Solution
Iodine is necessary for the synthesis of thyroid hormones, which regulate metabolism.
Correct Answer:
A
— Iodine
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Q. Which molecule acts as the electron carrier in glycolysis?
A.
NAD+
B.
FAD
C.
CoA
D.
ATP
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Solution
NAD+ acts as the electron carrier, being reduced to NADH during glycolysis.
Correct Answer:
A
— NAD+
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Q. Which molecule carries amino acids to the ribosome during translation?
A.
mRNA
B.
tRNA
C.
rRNA
D.
DNA
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Solution
tRNA (transfer RNA) carries amino acids to the ribosome during translation.
Correct Answer:
B
— tRNA
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Q. Which molecule is a key regulator of fatty acid oxidation?
A.
Insulin
B.
Glucagon
C.
Citrate
D.
Acetyl-CoA
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Solution
Glucagon is a key regulator that promotes fatty acid oxidation during fasting by activating lipolysis.
Correct Answer:
B
— Glucagon
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Q. Which molecule is commonly involved as a second messenger in cell signaling?
A.
ATP
B.
cAMP
C.
DNA
D.
RNA
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Solution
Cyclic AMP (cAMP) is a well-known second messenger that transmits signals from receptors to target molecules inside the cell.
Correct Answer:
B
— cAMP
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Q. Which molecule is produced as a byproduct of fatty acid oxidation?
A.
Lactate
B.
Acetyl-CoA
C.
Glucose
D.
Glycerol
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Solution
Acetyl-CoA is produced as a byproduct of fatty acid oxidation and can enter the citric acid cycle.
Correct Answer:
B
— Acetyl-CoA
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Q. Which molecule is the primary energy currency of the cell?
A.
Glucose
B.
ATP
C.
NADH
D.
FADH2
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Solution
Adenosine triphosphate (ATP) is the primary energy currency of the cell, used to store and transfer energy.
Correct Answer:
B
— ATP
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Q. Which molecule is the starting substrate for the TCA cycle?
A.
Pyruvate
B.
Acetyl-CoA
C.
Oxaloacetate
D.
Citrate
Show solution
Solution
Acetyl-CoA is the starting substrate for the TCA cycle, combining with oxaloacetate to form citrate.
Correct Answer:
B
— Acetyl-CoA
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