Biochemistry

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Q. Which enzyme is responsible for synthesizing RNA from a DNA template?
  • A. DNA polymerase
  • B. RNA polymerase
  • C. Ribosome
  • D. Ligase
Q. Which enzyme is responsible for the hydrolysis of lactose in E. coli?
  • A. Lactase
  • B. Beta-galactosidase
  • C. Lactose permease
  • D. Galactosidase
Q. Which factor can affect the resolution in HPLC?
  • A. Column length
  • B. Particle size of stationary phase
  • C. Flow rate
  • D. All of the above
Q. Which factor can affect the resolution of a gas chromatography separation?
  • A. Column length
  • B. Temperature of the detector
  • C. Type of carrier gas
  • D. All of the above
Q. Which factor can affect the yield of protein purification?
  • A. Temperature
  • B. pH
  • C. Choice of purification method
  • D. All of the above
Q. Which factor does NOT affect the separation in chromatography?
  • A. Temperature
  • B. Flow rate of the mobile phase
  • C. Nature of the stationary phase
  • D. Color of the sample
Q. Which hormone is primarily responsible for lowering blood glucose levels?
  • A. Glucagon
  • B. Insulin
  • C. Cortisol
  • D. Adrenaline
Q. Which immune cells are responsible for the destruction of virus-infected cells?
  • A. B cells
  • B. Helper T cells
  • C. Cytotoxic T cells
  • D. Regulatory T cells
Q. Which immune response is primarily activated by antigens?
  • A. Innate immune response
  • B. Adaptive immune response
  • C. Inflammatory response
  • D. Allergic response
Q. Which immunological technique is used to separate proteins based on their size?
  • A. Western blotting
  • B. Flow cytometry
  • C. Immunofluorescence
  • D. PCR
Q. Which immunological technique uses fluorescent dyes to visualize cells?
  • A. Immunofluorescence
  • B. ELISA
  • C. Western blotting
  • D. PCR
Q. Which level of protein structure is affected by denaturation?
  • A. Primary structure
  • B. Secondary structure
  • C. Tertiary structure
  • D. All of the above
Q. Which lipid is known as 'bad cholesterol'?
  • A. HDL
  • B. LDL
  • C. VLDL
  • D. Triglycerides
Q. Which macromolecule is involved in the storage of genetic information?
  • A. Proteins
  • B. Nucleic acids
  • C. Carbohydrates
  • D. Lipids
Q. Which macromolecule is primarily responsible for the structure of cell membranes?
  • A. Proteins
  • B. Nucleic acids
  • C. Carbohydrates
  • D. Lipids
Q. Which macromolecule serves as the primary source of energy for cells?
  • A. Proteins
  • B. Nucleic acids
  • C. Carbohydrates
  • D. Lipids
Q. Which metabolic pathway is primarily responsible for the detoxification of ammonia in the liver?
  • A. Glycolysis
  • B. Urea cycle
  • C. Krebs cycle
  • D. Fatty acid oxidation
Q. Which method can be used to determine the purity of a protein sample?
  • A. Mass spectrometry
  • B. Western blotting
  • C. Both A and B
  • D. None of the above
Q. Which mineral is a key antioxidant that helps protect cells from damage?
  • A. Selenium
  • B. Iron
  • C. Copper
  • D. Zinc
Q. Which mineral is crucial for maintaining healthy bones and teeth?
  • A. Sodium
  • B. Potassium
  • C. Calcium
  • D. Iron
Q. Which mineral is essential for the synthesis of collagen?
  • A. Zinc
  • B. Iron
  • C. Calcium
  • D. Sodium
Q. Which mineral is important for nerve transmission?
  • A. Calcium
  • B. Iron
  • C. Potassium
  • D. Sodium
Q. Which mineral is important for thyroid hormone production?
  • A. Iodine
  • B. Zinc
  • C. Calcium
  • D. Magnesium
Q. Which molecule acts as the electron carrier in glycolysis?
  • A. NAD+
  • B. FAD
  • C. CoA
  • D. ATP
Q. Which molecule carries amino acids to the ribosome during translation?
  • A. mRNA
  • B. tRNA
  • C. rRNA
  • D. DNA
Q. Which molecule is a key regulator of fatty acid oxidation?
  • A. Insulin
  • B. Glucagon
  • C. Citrate
  • D. Acetyl-CoA
Q. Which molecule is commonly involved as a second messenger in cell signaling?
  • A. ATP
  • B. cAMP
  • C. DNA
  • D. RNA
Q. Which molecule is produced as a byproduct of fatty acid oxidation?
  • A. Lactate
  • B. Acetyl-CoA
  • C. Glucose
  • D. Glycerol
Q. Which molecule is the primary energy currency of the cell?
  • A. Glucose
  • B. ATP
  • C. NADH
  • D. FADH2
Q. Which molecule is the starting substrate for the TCA cycle?
  • A. Pyruvate
  • B. Acetyl-CoA
  • C. Oxaloacetate
  • D. Citrate
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