Sterilization and Disinfection - Real World Applications
Q. In which scenario would you use a high-level disinfectant?
A.For cleaning floors
B.For sterilizing surgical instruments
C.For disinfecting semi-critical items like endoscopes
D.For routine hand hygiene
Solution
High-level disinfectants are used for semi-critical items that come into contact with mucous membranes, such as endoscopes, where sterilization is not feasible.
Correct Answer: C — For disinfecting semi-critical items like endoscopes
Q. What is the primary mechanism of action for quaternary ammonium compounds as disinfectants?
A.Oxidation of cellular components
B.Disruption of cell membranes
C.Inhibition of protein synthesis
D.Interference with nucleic acid synthesis
Solution
Quaternary ammonium compounds primarily act by disrupting cell membranes, leading to cell lysis and death of the microorganism.
Correct Answer: B — Disruption of cell membranes
Q. What is the role of autoclaving in sterilization?
A.It uses chemical agents to kill microorganisms.
B.It uses high pressure and steam to achieve sterilization.
C.It relies on ultraviolet light to disinfect surfaces.
D.It is a method of physical disinfection only.
Solution
Autoclaving uses high pressure and steam to achieve sterilization, effectively killing all microorganisms, including spores.
Correct Answer: B — It uses high pressure and steam to achieve sterilization.
