Biotechnology Applications: PCR, Cloning, Recombinant DNA - Real World Applications
Q. What is a common application of gene cloning?
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A.
Producing insulin
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B.
Creating vaccines
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C.
Gene therapy
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D.
All of the above
Solution
Gene cloning is used in various applications, including producing insulin, creating vaccines, and gene therapy.
Correct Answer: D — All of the above
Q. What is the main advantage of using plasmids as vectors in cloning?
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A.
They are large and complex
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B.
They replicate independently of chromosomal DNA
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C.
They are difficult to manipulate
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D.
They do not carry antibiotic resistance genes
Solution
Plasmids can replicate independently of chromosomal DNA, making them ideal vectors for cloning.
Correct Answer: B — They replicate independently of chromosomal DNA
Q. What is the significance of the 'sticky ends' in recombinant DNA technology?
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A.
They help in DNA replication
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B.
They facilitate the ligation of DNA fragments
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C.
They are used for PCR amplification
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D.
They stabilize the DNA structure
Solution
'Sticky ends' are overhanging sequences that allow for the easy ligation of DNA fragments during recombinant DNA technology.
Correct Answer: B — They facilitate the ligation of DNA fragments
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