Biotechnology Applications: PCR, Cloning, Recombinant DNA - Problem Set
Q. In cloning, what is the purpose of using a vector?
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A.
To provide a means of introducing foreign DNA into a host cell
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B.
To degrade unwanted DNA
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C.
To amplify RNA
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D.
To sequence DNA
Solution
Vectors are used to introduce foreign DNA into host cells, allowing for replication and expression of the inserted gene.
Correct Answer: A — To provide a means of introducing foreign DNA into a host cell
Q. What is the primary function of PCR (Polymerase Chain Reaction)?
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A.
To amplify DNA sequences
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B.
To sequence DNA
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C.
To cut DNA at specific sites
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D.
To synthesize RNA
Solution
PCR is used to amplify specific DNA sequences, making millions of copies of a particular segment.
Correct Answer: A — To amplify DNA sequences
Q. What is the role of restriction enzymes in molecular biology?
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A.
To cut DNA at specific sequences
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B.
To synthesize RNA
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C.
To amplify DNA
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D.
To repair DNA
Solution
Restriction enzymes cut DNA at specific sequences, which is essential for cloning and recombinant DNA technology.
Correct Answer: A — To cut DNA at specific sequences
Q. What is the significance of gel electrophoresis in molecular biology?
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A.
To separate DNA fragments based on size
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B.
To amplify DNA
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C.
To synthesize proteins
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D.
To sequence RNA
Solution
Gel electrophoresis is used to separate DNA fragments based on their size, allowing for analysis and comparison.
Correct Answer: A — To separate DNA fragments based on size
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