Biotechnology Applications: PCR, Cloning, Recombinant DNA - Competitive Exam Level
Q. What is the function of RNA polymerase during transcription?
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A.
To replicate DNA
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B.
To synthesize RNA from a DNA template
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C.
To splice RNA
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D.
To translate mRNA into protein
Solution
RNA polymerase synthesizes RNA by transcribing the genetic information from a DNA template.
Correct Answer: B — To synthesize RNA from a DNA template
Q. Which of the following is a common application of cloning in biotechnology?
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A.
Gene therapy
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B.
DNA sequencing
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C.
Protein purification
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D.
All of the above
Solution
Cloning is used in various applications, including gene therapy, DNA sequencing, and protein purification.
Correct Answer: D — All of the above
Q. Which technique is used to separate DNA fragments based on size?
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A.
PCR
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B.
Gel electrophoresis
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C.
Cloning
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D.
DNA sequencing
Solution
Gel electrophoresis is used to separate DNA fragments according to their size, allowing for analysis and comparison.
Correct Answer: B — Gel electrophoresis
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